The current work focuses on the synthesis and characterization of 2-quinolone-substituted hydrazide derivatives via Schiff base formation. The hydrazide precursor was first prepared, then condensed with appropriately substituted aldehydes in ethanol under acidic conditions to yield the desired Schiff bases. The reaction proceeded smoothly and produced high yields, confirming the efficacy of the synthetic protocol. The newly synthesized compounds were characterized by spectroscopic techniques such as FT-IR, 1H-NMR, 13C-NMR, mass spectrometry, which confirmed the formation of the imine (–C=N–) linkage. The presence of significant functional groups and structural features validated the successful synthesis of the target molecules. These derivatives are of great interest due to their potential biological activities, such as antibacterial and antifungal, anthelmintic, anti-inflammatory, and antioxidant properties.