A porous carbon anchored nickel molybdate (NiMoO4) nanoflower nanocomposite was successfully synthesized through a simple hydrothermal method for electrochemical sensing applications. The prepared nanocomposite was characterized using UV-Visible spectroscopy, FTIR, and XRD analysis to investigate its optical properties, functional groups, and crystalline nature. The synthesized material exhibited a flower-like morphology with enhanced electrochemical activity due to the synergistic interaction between porous carbon and NiMoO4 nanostructures. The electrochemical sensing performance of the modified electrode was evaluated toward L-tryptophan detection under different experimental conditions. The sensor showed excellent sensitivity with an optimum response at pH 4. The developed sensor achieved a low limit of detection (LOD) of 0.06 µM and a limit of quantification (LOQ) of 0.2 µM, indicating its potential for sensitive biomolecule sensing applications. These results demonstrate that the porous carbon anchored NiMoO4 nanoflowers are promising electrode materials for electrochemical sensing platforms