A series of 2-Amino-6-bromopyridine derivatives were synthesized through a multi-step reaction sequence. In addition, the generated compounds were characterised via spectroscopically (HPLC, 1 H NMR, 13 C NMR, Mass, & IR). Further, the confirmed compounds were tested to identify their efficacy as antibacterial and antifungal hits over Enterococcus and Trichoderma. As a result, few compounds revealed significant activity. On the other hand, in silico pharmacokinetic property screening was performed by using software ADMET 2.O and PkCSM. Subsequently, docking studies was performed to assess their binding affinity against the protein (PDB ID: 1QNR & 5IBY. Conclusively, the activity and in-silico results were corelating with each other. In further, additional modification on these molecules may help to investigate the potent lead over