CuO/Cr₂O₃ nanocomposite was successfully synthesized via the sol–gel method and systematically characterized to investigate its structural, morphological, and optical properties. The crystalline structure and average crystallite size of the synthesized nanocomposite were confirmed by X-ray diffraction (XRD) analysis, revealing a crystallite size of 40 nm. Surface morphology and elemental composition were examined through Scanning Electron Microscopy (SEM) and Energy Dispersive X- ray Analysis (EDAX), respectively. Optical properties were analyzed using UV–Visible Diffuse Reflectance Spectroscopy (UV–DRS). The electrochemical sensing performance of the synthesized nanocomposite was evaluated by modifying a carbon paste electrode (MCPE) for tyrosine detection using cyclic voltammetry (CV). The fabricated sensor exhibited remarkable electrocatalytic activity with high sensitivity toward tyrosine, achieving a detection limit of 4.95 × 10⁻⁶ M. Furthermore, the influence of scan rate, analyte concentration, and pH on sensing performance was systematically investigated to optimize the electrochemical response. The obtained results demonstrate that the CuO/Cr₂O₃ nanocomposite is a promising electrode material for efficient electrochemical tyrosine sensing.