The Sol-gel synthesized Co3O4/NiFe2O4 nanocomposite exhibited a crystallite size of ~26 nm and was comprehensively characterized to confirm its structural and surface properties. To evaluate its sensing capability, the synthesized nanocomposite was incorporated into a modified carbon paste electrode (MCPE). L-Tyrosine (L-TY) was investigated electrochemically using scan rate variation, which has verified that the analyte exhibit diffusion-controlled process. The fabricated sensor demonstrated significant electrocatalytic activity toward L-TY oxidation with a detection limit of 17.25 × 10⁻6 M, highlighting its suitability for electrochemical sensing applications.